Vaccinia virus expression and sequence of an avian influenza nucleoprotein gene: potential use in diagnosis
Identifieur interne : 002111 ( Main/Exploration ); précédent : 002110; suivant : 002112Vaccinia virus expression and sequence of an avian influenza nucleoprotein gene: potential use in diagnosis
Auteurs : V. R. Harley ; P. J. Hudson ; B. E. H. Coupar [Australie] ; P. W. Selleck [Australie] ; H. Westbury [Australie] ; D. B. Boyle [Australie]Source :
- Archives of Virology [ 0304-8608 ] ; 1990-03-01.
English descriptors
- Teeft :
- Antigenic variation, Avian, Avian influenza, Avian influenza strain, Cdna clone, Comparable amounts, Confluent monolayers, Disease control, Elisa, Harley, High backgrounds, Horse radish peroxidase, Human influenza, Influenza, Influenza virus, Monoclonal, Monoclonal antibodies, Monoclonal antibody, Nucleoprotein, Optimised dilution, Polyclonal, Polyclonal antisera, Polyclonal sera, Proc natl acad, Rabbit serum, Recombinant, Recombinant vaccinia virus, Recombinant virus, Sandwich elisa, Standard methods, Vaccinia, Vaccinia virus, Vaccinia virus expression, Virus, Virus strain, Western blot analysis.
Abstract
Summary: The nucleoprotein (NP) gene from avian influenza strain A/Shearwater/Aust/1/72 (H6N5) was cloned, sequenced, and expressed in vaccinia virus for the production of potent sera in immunised rabbits. The NP gene is 1565 bp and shares >95% amino acid sequence identity with other NPs of the avian subtype. The recombinant NP expressed by vaccinia virus comigrated with endogenous A/Shearwater/Aust/1/72 NP by Western blot analysis. Polyclonal rabbit sera raised against recombinant NP was evaluated in an antigen capture ELISA system as a potential diagnostic tool for the detection of avian influenza. All type A strains, comprising several HA and NA subtypes, but not type B nor other avian viruses, were detected.
Url:
DOI: 10.1007/BF01318362
Affiliations:
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The NP gene is 1565 bp and shares >95% amino acid sequence identity with other NPs of the avian subtype. The recombinant NP expressed by vaccinia virus comigrated with endogenous A/Shearwater/Aust/1/72 NP by Western blot analysis. Polyclonal rabbit sera raised against recombinant NP was evaluated in an antigen capture ELISA system as a potential diagnostic tool for the detection of avian influenza. All type A strains, comprising several HA and NA subtypes, but not type B nor other avian viruses, were detected.</div></front></TEI><affiliations><list><country><li>Australie</li></country></list><tree><noCountry><name sortKey="Harley, V R" sort="Harley, V R" uniqKey="Harley V" first="V. R." last="Harley">V. R. Harley</name><name sortKey="Hudson, P J" sort="Hudson, P J" uniqKey="Hudson P" first="P. J." last="Hudson">P. J. Hudson</name></noCountry><country name="Australie"><noRegion><name sortKey="Coupar, B E H" sort="Coupar, B E H" uniqKey="Coupar B" first="B. E. H." last="Coupar">B. E. H. 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